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引物特异性可以通过执行BLAST搜索和从程序内部搜索模板结构来实现。这两种搜索结果都用于设计引物。引物设计过程中，会自动避免出现显著交叉同源性和模板结构的区域。
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Three multiplex snapshot assays for SNP genotyping in candidate innate immune genes
Lisa M Esteves, Sara M Bulhoes, Maria J Brilhante, Luisa Mota-Vieira.
BMC Research Notes. 2013; Feb 7;6:. doi: 10.1186/1756-0500-6-.
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